Evaluation of Growth Parameters on Extracellular Lipase Enzyme Production by Staphylococcus Aureus

Lipases have the ability to perform biotransformation which has made them increasingly popular in the detergent, food, chemical, and pharmaceutical industries. Environmental pollution due to oil effluents is one of the major problems and there is need for detoxification and destruction of these contaminants effluents. Different Lipase producing microorganisms are used for the oil effluent remediation process to detoxify and degrade the oil effluents. In the present investigation, bacteria producing extracellular lipase were isolated from industrial effluents and identified as Staphylococcus aureus by morphological, biochemical and Molecular characterization. Growth of the organisms and lipase production were measured with varying pH (4-10), incubation temperature (4 to 60 C), incubation time, various nitrogen, carbon and substrate sources. The composition of the medium and changes in pH, temperature and incubation period affected the amount of enzyme produced. Lipase activity was maximum at pH 7 for incubation period of 48 hours at 35o C and results in highest lipid activity in the media containing glycerol as carbon, casein as nitrogen and cod liver oil as substrate source. The present work describes the comparative study on the effect of different growth media and conditions on lipase production by Staphylococcus aureus.